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FORSCHUNGSBERICHT 1996-1998


 

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Institut für Physiologische Chemie

Allgemeine Angaben:
Nußallee 11, 53115 Bonn, Telefon: 0228 / 73-2411 /12, Telefax: 0228 / 73-2416, eMail: phch@institut.physiochem.uni-bonn.de

Hauptamtliche Professoren
Prof. Dr. rer. nat. Ernst Bause
Prof. Dr. rer. nat Heinz Egge
Prof. Dr. rer. nat. Manfred Kempfle

Wissenschaftliches Personal
Universitätsstellen
Dr. rer. nat Erhard Bieberich
Dr. rer. nat. Wilhelm Breuer
Dipl.-Chem. Martina Dieckmann
Dipl.-Chem. Simone Glomsda
Dr. rer. nat. Heinz Rudolf Hartmann
Dr. rer. nat. Sabine Homfeld
Dr. rer. nat. Robert Hurwitz
Dr. rer. nat. Hem Chandra Jha
Dipl.-Chem. Saeed Khoursandi
Dr. phil. (Ph.D.) Roger A. Klein
AOR Prof. Dr. rer. nat. Uwe Murawski
Dr. rer. nat. Jasna Peter-Katalinic
PD Dr. rer. nat. Gottfried Pohlentz
Dr. rer. nat. Eva Schaffner
Dipl.-Chem. Andreas Schmitt
Dipl.-Oec.troph. Susanne Starcke
Dr. rer. nat. Christian Vogel
Dr. rer. nat. Christof Völker

Drittmittelstellen
Dr. rer. nat. Hardy Arends ()
Dipl.-Chem. Achim Bartoschek ()
Dipl.-Chem. Benno Coßmann ()
Dipl.-Chem. Birgit Hardt ()
Dr. rer. nat. Markus Hoppe ()
Dr. rer. nat. Christoph Kaes ()
Dr. rer. nat. Burga Kalz-Füller ()
Dr. rer. nat. Serafim Kiriakidis ()
Dr. rer. nat. Iris Meisen ()
Dipl.-Phys. Victor Pacheco-Torres ()
Bipl.-Biol. Kornelia Treml ()

Stipendiaten
Dipl.-Chem. Raquel Aparicio ()

Forschungsschwerpunkte
Katalysemechnismus und Spezifität von Oligosaccharyltransferasen
(Bause, Breuer, Hardt, Homfeld, Bartoschek)

Struktur und Funktion von Glykosidasen des "frühen" Oligosaccharid-processings
(Bause, Aparicio, Bieberich, Hentges, Kalz-Füller, Treml, Völker)

Glykosylphosphatidylinositol-(GPI)-Anker und GPI-spezifische Phospholipasen ausTrypanosoma brucei brucei
(Egge, Hurwitz, Klein)

Struktur und Konformationsanalyse von Glykokonjugaten, Kohlenhydrat-Protein-Wechselwirkungen mit Hilfe der NMR-Spektroskopie und Computerberechnungen
(Egge, Klein, Hartmann)

Untersuchungen an und mit 4,6,8(14)-Trien-3-on Steroiden
(Kempfle, Schmitt)

Untersuchungen des Metabolismus von ETO und CHT in Rattenlebermikrosomen
(Kempfle)

Dünnschicht- und Gaschromatographie von Lipiden und Fettsäuren unterschiedlicher Organe der Wüstenrennmaus
(Murawski)

Charakterisierung von Glykosyltransferasen des Golgi mit Hilfe von Neoglykolipiden
(Pohlentz, Kaes)

Besondere Forschungsförderung
Aktives Zentrum, Aufbau und Membrantopologie des Oligosaccharyltransferase-Komplexes aus Schweineleber
(Bause, Bartoschek, Breuer, Hardt, Homfeld, SFB 284, Teilprojekt A2)

Enzymologie und Molekularbiologie von processing-Glykosidasen
(Bause, Bieberich, Hentges, Kalz-Füller, Treml, Völker, SFB 284, Teilprojekt B3)

Tempe-Fermentation: Isolierung und Strukturaufklärung der sekundären Metaboliten des Tempe und Untersuchung der physiologisch-chemischen Wirkungen dieser Substanzen
(Egge, Jha, F+E-Vertrag 931/105216/32 (Biotechnology Indonesia - Germany / BMBF-TÜV Rheinland))

European Network on Biomolecular Mass Spectrometry
(Egge, EEC Contract Human Capital and Mobility CHRX-CT 930283 )

Charakterisierung und Lokalisierung GPI-spaltender Enzyme in "Trypanosoma brucei brucei"
(Hurwitz, SFB 284, Teilprojekt B7)

Structural and conformational analysis of glycoconjugates
(Klein, Hartmann, SFB 284, Teilprojekt A1)

High sensitive - high mass spectrometry in the structural studies of biomolecules
(Pohlentz, EEC-CRN Contract GE1 CT92-0045 )

Reinigung und Charakterisierung von Glykosyltransferasen aus Rattenleber mit Hilfe von Neoglykolipiden vom 1-Deoxy-1-phosphatidylethanolamino-lactitol
(Pohlentz, SFB 284, Teilprojekt A5)

Veröffentlichungen
Albrecht B., Pohlentz G., Sandhoff K., Schwarzmann G.:
Synthesis and mass spectrometric characterization of digoxigenin and biotin labeled ganglioside GM1 and their uptakeby and metabolism in cultured cells.
Chem. Phys. Lipids 86, 37-50, 1997

Barreto-Bergter E., Branquinha M.H., Pohlentz G., Vermelho A.B.:
Monohexosylceramides of Trypanosoma dionisii.
J.Eukaryot. Microbiol. 46, 486-488, 1996

Bause E., Wesemann M., Bartoschek A., Breuer W.:
Epoxyethylglycylpeptides as inhibitors of oligosaccaryltransferase: double-labelling of the active site.
Biochem. J. 332, 95-102, 1997

Bause E., Burbach M.:
Purification and enzymatic properties of endo-a1,2-mannosidase from pig liver involved in oligosaccharide processing.
Biol. Chem. Hoppe Seyler 377, 639-646, 1996

Bieberich E., Treml K., Völker C., Rolfs A., Kalz-Füller B., Bause E.:
Man9-mannosidase from pig liver is a type-II membrane protein that resides in the endoplasmic reticulum:cDNA cloning and expression of the enzyme in COS 1 cells.
Eur. J. Biochem. 246, 681-689, 1997

Cheng D.-L., Shao Y., Hartmann R., Roeder E.:
Pentapeptides from the roots of Aster tataricus.
Pharmazie 51, 185-186, 1996

da Silva Bahia M.C.F., Vieira R.P., Mulloy B., Hartmann R., Barreto-Bergter E.:
Thestructure of polysaccharides and glycolipids of Aspergillus fumigatus grown in the presenceof human serum.
Mycopathologia 137, 17-25, 1997

Duarte R.S., Polycarpo C.R., Wait R., Hartmann R., Barreto-Bergter E.:
Structural characterization of neutral glycosphingolipids from Fusarium species.
Biochim. Biophys. Acta 1390, 186-196, 1998

Hanisch F.G., Stadie T.R.E., Deutzmann F., Peter-Katalinic J.:
MUC1 glycoforms in breast cancer. Cell line T47D as a model for carcinoma-associated alterations of O-glycosylation.
Eur. J. Biochem. 236, 318-327, 1996

Hentges A., Bause E.:
Affinity purification and characterization of glucosidase II from pig liver.
Biol. Chem. 378, 1031-1038, 1997

Hilpisch U., Hartmann R., Glombitza K.W.:
New dammaranes, esterified with melonic acid from leaves of betula pendula.
Planta Med. 63, 347-351, 1997

Hoppe M.B., Jha H.C., Egge H.:
Structure of an antioxidant from fermented soybeans (tempe).
J. Am. Oil Soc. 74/4, 477-479, 1997

Jonsdottir S.O., Klein R.A., Rasmussen K:
UNIQUAC interaction parameters for alkane/amine systems determined by molecular mechanics.
Fluid Phase Equilibria 11, 59-72, 1996

Jonsdottir S.O., Klein R.A.:
UNIQUAC interaction parameters for molecules with OH-groups on adjacent carbon atoms in aqueous solution determined by molecular mechanics -glycols, glycerol and glucose.
Fluid Phase Equilibria 132, 117-137, 1997

Kalz-Füller B., Heidrich-Kaul C., Nöthen M., Bause E., Schwanitz, G.:
Localization of the human glucosidase I gene to chromosome 2p12-p13 by fluorescence in situ hybridization and PCR analysis of somatic cell hybrides.
Genomics 34, 442-443, 1996

Kiriakidis S., Stathi S., Jha H.C., Hartmann R., Egge H.:
Fatty acid esters of sitosterol3ß-glucoside from soybeans and tempe (fermented soybeans) as antiproliferative substances.
J. Clin. Biochem. Nutr. 22, 139-147, 1997

Klein R.A., Hartmann R., Egge H., Behr T., Fischer W.:
The aqueous solution structure of a lipoteichoic acid from Streptococcus pneumoniae strain R6 containing 2,4-diamino-2,4,6-trideoxy-galactose: evidence for conformational mobility of the galactopyranose ring.
Carbohyd. Res. 281, 79-98, 1996

Klein R.A., Jonsdottir S.O., Egge H.:
Aspects of conformational mobility in charged oligosaccharides.
J. Mol. Struc. 395/96, 313-332, 1997

Kniep B., Peter-Katalinic J., Müthing J., Majdic O., Pickl W.F., Knapp W.:
The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer.
J. Biochem. Tokyo 119, 456-462, 1996

Lansmann S., Ferlinz K., Hurwitz R., Bartelsen O., Glombitza G., Sandhoff K.:
Purification of the acid sphingomyelinase from human placenta: Characterization and N-terminal sequence.
FEBS Lett. 399, 227-231, 1996

Mahran G.E.D.H., Glombitza K.W., Mirhom Y.W., Hartmann R., Michel C.G.:
Novel saponins from Zizyphus spina-christi growing in Egypt.
Planta Med. 62, 163-165, 1996

Müthing J., Spanbroek R., Peter-Katalinic J., Hanisch F.G., Hanski Ch., Hasegawa A., Unland F., Lehmann J., Tschesche H., Egge H.:
Isolation and structrual characterization of fucosylated gangliosides with linear poly-N-acetyllactosaminyl chains from human granulocytes.
Glycobiology 6, 147-156, 1996

Peter-Katalinic J.:
Bacterial carbohydrate antigens: From primary structure to non- covalent interactions.
Croat. Chem. Acta 69, 731-739, 1996

Pohlentz G., Marlis I., Egge H.:
Studies on fast atom bombardment of aminosugars using synthetic derivatives of the trisaccharide unit ß-D-glucopyranosyl-(1-3)-O-2-deoxy-2-amino-ß-D-glucopyranosyl-(1-4)-O-ß-D-glucopyranose.
J. Carbohyd. Chem. 17, 1151-1165, 1998

Pohlentz G., Mokros A., Egge H.:
Cholesterol-containing lactose derived neoglycolipids serve as acceptors for sialyltransferases from rat liver golgi vesicles.
Glycoconjugate J. 13, 147-152, 1996

Rudloff S., Pohlentz G., Dieckmann L., Egge H., Kunz C.:
Urinary excretion of lactose and oligosaccharides in preterm infants fed human milk or infant formula.
Acta Paediatr. 85, 598-603, 1996

Schaffner E.M., Hartmann R., Taraz K., Budzikiewicz H.:
Structure elucidation of azotobactin 87, isolated from Azotobacter vinelandii ATCC 12837.
Z. Naturforsch. 51c, 139-150, 1996

Spitzer V., Tomberg W., Hartmann R., Aichholz R.:
Analysis of the seed oil of Heisteria silvanii (Olacaceae) - A rich source of novel C18 acetylenic fatty acid.
Lipids 32, 1189-1200, 1997

Vermelho A.B., de Nazareth Leal de Mereilles M., Pereira M.C., Pohlentz G., Barreto-Bergter E.:
Heart muscle cells share common neutral glycosphingolipids with Trypanosoma cruzi.
Acta Tropica 64, 131-143, 1997

Vielhaber G., Hurwitz R., Sandhoff K.:
Biosynthesis, processing and targeting of SAP-precursor in human fibroblasts: Mannose-6-phosphate receptor independent endocytosis of SAP-precursor.
J. Biol. Chem. 271, 32438-32446, 1996


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