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FORSCHUNGSBERICHT 1999-2001

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Institut für Zellbiologie

Allgemeine Angaben:
Ulrich-Haberland-Straße 61 a, 53121 Bonn
Telefon: 0228 / 73-5301
Fax: 0228 / 73-5302
eMail: herzog@uni-bonn.de
WWW: http://www.uni-bonn.de/zellbiologie

Hauptamtliche Professoren:
Professor Dr. Albert Haas
Professor Dr. Volker Herzog
Professor Dr. Jörg Höhfeld
Professor Dr. Hans Komnick

Wissenschaftliches Personal:
Universitätsstellen
Privat-Dozentin Dr. Klaudia Brix
Dr. Claudia Elfgang
Dr. Gregor Kirfel
Dr. Peter Lemansky
Dr. Anton Schmitz
Dr. Rolf Stiemerling
Dr. Ritva Tikkanen

Drittmittelstellen
Dipl.-Biol. Simon Alberti (HO1518/5-1)
Dipl.-Biol. Peter Beicht (SFB 284)
Dipl.-Biol. Claudia Esser (HO 1518/4-1)
Dipl.-Biol. Ingo Gestmann (SFB 284)
Dipl.-Biol. Jens Hoffmann (SFB 284)
Dipl.-Biol. Ann Icking (SFB 284)
Dipl.-Biol. Markus Klein (SFB 284)
Dipl.-Biol. Martin Linke (SFB 284)
Dipl.-Biol. Anja Lührmann (DFG)
Dipl.-Biol. Thomas Quast (FOR 367/1)
Dipl.-Biol. Manuela Saathoff (FOR 367/1)
Dipl.-Biol. Anja Schüttfort (DFG)
Dipl.-Biol. Carmen Tepel (Zeiss)
Dipl.-Biol. Sven Wehner (Industrie)
Dipl.-Biol. Alexandra Winkeler (SFB 284)

Stipendiaten
Dipl.-Biol. Eugenia Fernandez-Mora (DAAD)

Forschungsschwerpunkte:
Molekulare Analyse der Phagosomenreifung in infizierten phagozytischen Zellen
(Haas)

Epitheliale Morphogenese
(Herzog)

Oligomerisierung und Multimerisierung sekretorischer Proteine
(Herzog)

"Zelluläre Mechanismen der Alzeimer Erkrankung"
(Herzog, Schmitz)

Proteineinfaltung und Proteinabbau
(Höhfeld)

Intestinale Verdauung und Absorption von Triglyceriden und Phospholipiden bei Insekten
(Komnick)

Besondere Forschungsförderung:
Bonner Forum Biomedizin
(Herzog, Ministerium für Schule und Weiterbildung, Wissenschaft und Forschung NRW)

DFG-Normalverfahren "Phagosom-Lyosom-Fusion in Makrophagen und ihre Hemmung durch pathogene Mikroorganismen"
(Haas, DFG)

DFG-Schwerpunkt "Struktur, Funktion und Regulation des 20S/26S Ubiquitin-Proteasomsystems"
(Höhfeld, DFG)

DFG-Schwerpunkt "Zelluläre Mechanismen der Alzheimer Erkrankung"
(Herzog, Schmitz, DFG)

Forschergruppe "Keratinocyten -Proliferation und differenzierte Leistung in der Epidermis"
(Herzog, DFG)

Graduiertenkolleg "Funktionelle Proteindomänen"
(Herzog, DFG)

Research Award "Biogenesis of phagosomes containing Legionella pneumophila"
(Haas, AGTI Inc.)

Sonderforschungsbereich 284 ("Glykokonjugate und Kontaktstrukturen der Zelloberfläche", Teilprojekte B1 und B2
(Herzog, Brix, Elfgang, Lemansky, Schmitz, Tikkanen, DFG)

Veröffentlichungen:

Bauer C, Herzog V, Bauer FB
Yeast Membrane Structure
Microscopy and Microanalysis 7, 530-534, 2001

Brix, K, Linke, M, Tepel, C, Herzog, V
Cysteine Proteinases Mediate Extracellular Prohormone Processing in the Thyroid.(Review).
Biol. Chem. 282, 717-725, 2001

Connell P, Ballinger CA, Jiang J, Wu Y, Thompson LJ, Höhfeld J, Patterson C
Regulation of heat shock protein-mediated protein triage decisions by the co-chaperone CHIP.
Nat. Cell. Biol. 3, 93-96, 2001

Demand J, Alberti S, Patterson C, Höhfeld J
cooperation of a ubiquitin domain protein and an E3 ubiquitin ligase during chaperone/proteasome coupling.
Curr. Biol. 11, 1569-1577, 2001

Dombrowski, F, Klotz, L, Hacker, HJ, Li, Y, Klingmuller, D, Brix, K., Herzog, V., Bannasch, P.
Hyperproliferative hepatocellular alterations after intraportal transplantation of thyroid follicles
Am. J. Pathol. 156, 99-113, 2000

Eitzen GA, Will E , Gallwitz D , Haas A, Wickner W
Sequential action of two GTPases to promote vacuole docking and fusion.
EMBO J. 19: 6713-6720, 2000

Hinz, B, Alt, W, Johnen, C, Herzog, V, Kaiser, HW
Quantifying lamella dynamics of cultured cells by SACED, a new computer-assisted motion analysis
Exp. Cell Res. 251, 234-243, 1999

Hitzel, C, Kanzler, H, Konig, A, Kummer, MP, Brix, K, Herzog, V., Koch, N.
Thyroglobulin type-I-like domains in invariant chain fusion proteins mediate resistance to cathepsin L digestion.
FEBS Lett. 485, 67-70, 2000

Hoffmann, J, Twiesselmann, C, Kummer, MP, Romagnoli, P, Herzog, V
A possible role for the Alzheimer amyloid precursor protein in the regulation of epidermal basal cell proliferation.
Eur. J. Cell Biol. 79, 905-914, 2000

Höhfeld J, Cry DM, Patterson C
From the cradle to the grave: molecular chaperones that may choose between folding and degradation.
EMBO Rep. 2, 885-890, 2001

Jiang J, Ballinger CA, Wu Y, Dai Q, Cry DM, Höhfeld J, Patterson C
CHIP is a U-box-depentent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation.
J. Biol.Chem. 276, 42938-42944, 2001

King FW, Wawrzynow A, Höhfeld J, Zylicz M
Co-chaperones Bag-1, hop and Hsp40 regulate Hsc70 and Hsp90 interactions with wild -type or mutant p53.
EMBO J. 22,6297-6305, 2001

Kirfel G, Komnik H
Differential absorption and esterification of diethary long-chain fatty acids by larvae of the dragonfly, Aeshna cyanea
Arch. Insect. Biochem. and Physiol. 40, 183-193, 1999

Klein, M, Gestmann, I, Berndorfer, U, Schmitz, A, Herzog, V
The thioredoxin boxes of thyroglobulin: possible implications for intermolecular disulfide bond formation in the follicle lumen.
Biol. Chem. 381, 593-601, 2000

Kuklinski, S, Pesheva, P, Heimann, C, Urschel, S, Gloor, S, Graeber, S. Herzog, V., Pietsch, T., Wiestler, O.D., Probstmeier, R.
Expression pattern of galectin-3 in neural tumor cell lines
J. Neurosci. Res. 60, 45-57, 2000

Lemansky, P, Brix, K, Herzog, V
Subcellular distribution, secretion and posttranslational modifications of clusterin in thyrocytes.
Exp. Cell Res. 251, 147-155, 1999

Lüders J, Demand J , Höhfeld J
The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome.
J. Biol. Chem. 275, 4613-4617, 2000

Lüders J, Demand J, Papp O, Höhfeld J
Distinct isoforms of the cofactor BAG-1 differentially affect Hsc70's chaperone function.
J. Bio. Chem. 275, 14817-14823, 2000

Lührmann A, Haas A
A method to purify bacteria-containing phagosomes from infected macrophages.
Methods Cell Sci. 22: 329-341, 2000

Lührmann A , Streker K , Schüttfort A, Daniels JJD, Haas A
Afipia felis resides in a macrophage compartment that is disconnected from the endocytic pathway.
Proc. Natl. Acad. Sci. USA 98:7271-7276, 2001

Mayer A , Scheglmann D , Dove S , Glatz A , Wickner W , Haas A
Phosphatidylinositol-(4,5)-bisphosphate regulates two steps of homotypic vacuole fusion
Mol. Biol. Cell 11:807-817, 2000

Möbius, W, Herzog, V, Sandhoff, K, Schwarzmann, G
Intracellular distribution of a biotin-labeled ganglioside GM1 by immuno-electron microscopy after endocytosis in fibroblasts.
J. Histochem. Cytochem. 47, 1005-1014, 1999

Möbius, W, Herzog, V, Sandhoff, K, Schwarzmann, G
Gangliosides are transported from the plasma membrane to intralysosomal membranes as revealed by immuno-electron microscopy.
Bioscience Reports 19, 307-316, 1999

Nollen EAA, Kabakov AE, Brunsting JF, Kanon B, Höhfeld J, Kampinga HH
Modulation of in vivo Hsp70 chaperone activity by HIP and BAG-1.
J. Biol. Chem. 276, 4677-4682, 2001

Saber-Lichtenberg, Y, Brix, K, Schmitz, A, Heuser, JE, Wilson, JH, Lorand, L., Herzog, V.
Covalent cross-linking of secreted bovine thyroglobulin by transglutaminase
FASEB J. 14, 1005-1104, 2000

Schepers, U, Lemm, T, Herzog, V, Sandhoff, K
Characterization of regulatory elements in the 5'-flanking region of the GM2 activator gene.
Biol. Chem. 381, 531-544, 2000

Schmitz, A, Herrgen, H, Winkeler, A, Herzog, V
Cholera toxin is exported from microsomes by the Sec61p complex
J. Cell Biol. 148,1203-1212, 2000

Schneider B, Gross R , Haas A
Bordetella bronchiseptica and B. pertussis are localized to a lysosomal compartment in macrophages, but B. bronchiseptica survives due to its acid-insensitivity.
Infect. Immun. 68:7039-7048, 2000

Sondermann H, Scheufler C, Schneider C, Höhfeld J, Hartl FU, Moarefi I
Structure of a Bag/Hsc70 complex: convergent functional evolution of Hsp70 nucleotide exchange factors.
Sci 291, 1553-1557, 2001

Tepel, C, Brömme, D, Herzog, V, Brix, K
Cathepsin K in thyroid epithelial cells: sequence, localization and possible function in extracellular proteolysis of thyroglobulin.
J. Cell Sci. 113, 4487-4498, 2000

Terjung B, Worman HJ, Herzog V, Sauerbruch T, Spengler U
Differentiation of antineutrophil nuclear antibodys in inflammatory bowe and autoimmune liver disease from antineutrophil cytoplasmic antibodies (p-ANCA) using immunofluorescence microscopy.
Clin. Exp. Immunol., 126: 37-46, 2000

Wickner WT , Haas A
Yeast vacuole fusion: A window on organelle inheritance
Annu. Rev. Biochem. 69:247-275, 2000


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